Western blot analysis of HBA1 in extracts of K-562 cells. Samples were incubated with HBA1 Polyclonal antibody (Product # PA5-109803) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of HBA1 in K-562. Samples were incubated in polyclonal HBA1 antibody (Product # PA5-109803) using a dilution of 1:3000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
Supplier Page from Thermo Fisher Scientific for HBA1 Antibody